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KMID : 0043320070300091132
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Archives of Pharmacal Research
2007 Volume.30 No. 9 p.1132 ~ p.1137
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2,3,6-Tribromo-4,5-dihydroxybenzyl Methyl Ether Induces Growth Inhibition and Apoptosis in MCF-7 Human Breast Cancer Cells
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Lee Ji-Hyeon
Park Sang-Eun
Hossain Mohammad Akbar
Kim Min-Young
Kim Mi-Na
Chung Hae-Young
Choi Jae-Sue
Yoo Young-Hyun
Kim Nam-Deuk
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Abstract
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In this study, we investigated the effects of 2,3,6-tribromo-4,5-dihydroxybenzyl methyl ether (TDB), isolated from Symphyocladia latiuscula (marine red algae), on the proliferation of MCF- 7 human breast cancer cells. TDB treatment for 48 h inhibited cancer cell growth and induced DNA fragmentation. Furthermore, morphological characterizations such as apoptotic bodies and membrane blebs were shown by electronic microscopy. TDB-induced apoptosis in the MCF-7 cells was closely linked with the down-regulation of Bcl-2 protein expression and the cleavage of caspase-3 substrates, with poly(ADP-ribose) polymerase cleavage occurring by TDB treatment. TDB treatment also caused a marked increase in the level of p21WAF1/CIP1 protein in a p53-dependent manner. In addition, the upregulation of p21WAF1/CIP1 in the MCF-7 cells was related to a decrease in c-Myc protein in a dose-dependent manner. Based on our data, TDB is a good candidate for further evaluation as an effective chemotherapeutic agent, acting through the induction of apoptosis.
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KEYWORD
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2,3,6-Tribromo-4, 5-dihydroxybenzyl methyl ether, Cytotoxicity, Apoptosis, Human breast cancer cells
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